Combining whole transcriptomics, protein detection, and tissue morphological information on the same tissue section allows for in-depth spatial multi-omics discovery. Stereo-seq v1.3 mIF solution enables spatial visualization of multiple proteins in single-cell resolution on top of the unbiased whole transcriptome information on the same tissue section. Without affecting mRNA capturing, the additional detected protein information can be integrated with gene expression data for in-depth evaluation of valuable samples, and to parse complex pathological and physiological processes.