Transcriptomics mIF Solution

Stereo-seq mIF Product Note Stereo-seq Permeabilization Set User Manual Stereo-seq Transcritpomics Set for mIF User Manual

Solution Overview

Combing whole transcriptomics, protein detection, and tissue morphological information on the same tissue section allows for in-depth spatial multi-omics discovery. Studying cellular heterogeneity empowers the discovery of new biomarkers, identifying the cellular origin of important expressed proteins within healthy and abnormal tissue microenvironments. It also further advances immuno-oncology research by studying how immune cells respond to tumor development, and what are the genes and proteins that are involved in the regulation processes.

Stereo-seq transcriptomics mIF solution enables spatial visualization of multiple proteins in single-cell resolution on top of the unbiased whole transcriptome information on the same tissue slice. Without affecting mRNA capturing, the additional detected protein information can be integrated with gene expression data to in-depth evaluate valuable samples, and to parse complex pathological and physiological processes.

Product Highlight

High fidelity protein staining imaging for enhanced spatial protein identification

Detect multiple proteins with unbiased whole transcriptome simultaneously in fresh frozen and PFA fixed samples.

Flexibility in choosing antibodies based on user's research needs and project design.

Co-detection of protein and RNA in a single experiment for extensible applications in tissue and cellular type study.

Key Features

Study protein, unbiased high-resolution whole transcriptome information and tissue morphology on the same tissue
Discover new tissue biomarkers and molecular targets at a spatial scale with cellular specificity
Interrogate gene expression profiles and protein markers indifferent tumor regions to further explore tumor progressing
Characterize immune cells and immune repertoires within healthy tissue microenvironment vs TME
ldentify the spatial location of secreted proteins within thecell under different conditions

Demo Data

Mouse brain

Section size: ~16.3 mm²
Number of mapped spatial barcodes (CIDs) under tissue: ~29.5 M
Number of genes: 356 (bin 20, 20 × 20 DNB)

Operational Instructions

Mouse testis

Tissue area: 19.85 mm²
Total reads: 425.78 M
Valid CID Read: 334,493,140
Median gene type count: 560
Cell count:155,725
Total transcriptome: 226.07 M
Median MID count per cell: 634
Median gene type count per cell: 509
Chip size: 1 cm x 1 cm

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Workflow

Perform Stereo-seq in your own laboratory with Stereo-seq Kit Solutions

Product Number	Product	Configuration
Stereo-seq Permeabilization Set for Chip-on-a-slide V1.1
211SP11118	Stereo-seq Permeabilization Kit 211KP11118	8 RXN
	Stereo-seq Chip P Slide (1cm*1cm) 210CP118	8 EA
	STOmics Accessory Kit 1000033700	5 PCS
Stereo-seq Transcriptomics Set for Chip-on-a-slide V1.3
211ST13114	Stereo-seq Transcriptomics T Kit 211KT13114	4 RXN
	Stereo-seq Chip T Slide (1cm*1cm) 210CT13114	4 EA
	STOmics Accessory Kit 1000033700	5 PCS
111KL160	Stereo-seq 16 Barcode Library Preparation Kit	16 RXN
301AUX001	Stereo-seq PCR Adaptor	2 EA

Product Number

Product

Configuration

Stereo-seq Permeabilization Set for Chip-on-a-slide V1.1

Stereo-seq Transcriptomics Set for Chip-on-a-slide V1.3